Chapter 4
Enzymes and Bioenergetics
Exercises
1. In order to catalyse a reaction, an enzyme is required to
(a) be saturated with substrate
(b) decrease the activation energy
(c) increase the equilibrium constant
(d) increase the activation energy
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2. Pepsin is a gastric enzyme. Does it have an acidic or alkaline optimum pH? What happens to pepsin when it enters the duodenum?
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3. What is the relationship between vitamins and enzyme co-factors?
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4. What is the effect of temperature, pH and substrate concentration on catalytic activity of enzyme?
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5. The rate determining step of Michaelis-Menten kinetics is
(a) the complex dissociation of ES complex
(b) the complex formation
(c) the product formation
(d) the product degradation
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6. Define Km and its significance.
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7. What is meant by one unit of enzyme?
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8. What is specific activity of an enzyme?
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9. Briefly describe first and second laws of thermodynamics.
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10. Define entropy. What is the relationship between free energy and entropy?
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11. Why ATP is called universal energy currency?
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